《广州医药》
点击关注官方服务号追踪稿件状态

【重要服务提醒】《广州医药》杂志服务号已上线稿件查询功能

各位作者、审稿专家:
本刊官方微信服务号新增稿件状态查询服务,无需反复登录投稿系统,微信即可随时查看审稿进度、修回通知、录用结果,稿件更新实时推送提醒,同步接收期刊征稿、学术资讯。

操作方式

微信搜索关注广州医药杂志服务号 → 点击菜单栏「绑定账号」→ 输入投稿邮箱完成账号关联;请关闭微信消息免打扰,避免遗漏稿件通知。

《广州医药》编辑部

广州医药杂志服务号
您的位置: 首页 > 2015年3月 第46卷 第2期 > 文字全文
2023年7月 第38卷 第7期11
目录

急性肺损伤大鼠吸入一氧化氮后炎症介质变化

Effect of nitric oxide to acute lung injury mice and the changes of inflammatory factors and endothelial progenitor cells(EPCs)

来源期刊: 广州医药 | 4-6 发布时间:2021-11-30 收稿时间:2025/11/13 16:48:04 阅读量:99
作者:
关键词:
一氧化氮急性肺损伤内皮祖细胞髓过氧化酶白细胞介素-10(IL-10)
Nitric oxide(NO)ALIEPCsMPOIL-10
DOI:
10.3969/j.issn.1000-8535.2015.02.002
收稿时间:
2014-11-28 
修订日期:
 
接收日期:
 
引用总数:
0  
目的 观察并评估内毒素性急性肺损伤大鼠吸入一氧化氮后外周血中内皮祖细胞和炎症介质的变化情况。方法 90只SPF级健康大鼠分为3组,A组为正常对照组(n=30),B组为急性肺损伤组(ALI)(n=30), C组为一氧化氮(NO)组(n=30)。分别计算各组外周血内皮祖细胞(Endothelial progenitor cells,EPCs) 数量,同时监测肺组织中白细胞介素-10(Interleukin-10,IL-10)水平和髓过氧化物酶(Myeloperoxidase,MPO)活性。结果 我们成功建立了大鼠的ALI肺损伤模型, C组EPCs数量、MPO活性上升幅度均小于B组、而IL-10上升水平均高于B组,差异有统计学意义(P<0.05)。结论 大鼠吸入一氧化氮可减轻内毒素所致急性肺损伤程度,其机制可能与外周血中内皮祖细胞数量及MPO水平下降和IL-10水平上升有关。
Objective To investigate the effect of nitric oxide(NO) inhalation in endotoxin-induced acute lung injury mice. Methods Ninety SPF mice were randomly assigned to the normal group(group A), ALI group(group B)and ALI+NO group(group C). The number of endothelial progenitor cells was counted and the level of Interleukin-10(IL-10) and myeloperoxidase (MPO) were measured. Results Endotoxin administration resulted in pulmonary edema. The pulmonedema was lightened and the level of MPO were decreased by the inhalation of nitric oxide while the level of IL-10 increased. Conclusion NO inhalation can mitigate acute lung injure. The decline of EPCs and MPO and the increase of IL-10 may be one of the mechanism.
1、 Pipeling MR,Fan E.Theraperies for refractory hypoxemia in acute respiratory distress syndrome[J].JAMA,2010,304(22):2521-2527. Pipeling MR,Fan E.Theraperies for refractory hypoxemia in acute respiratory distress syndrome[J].JAMA,2010,304(22):2521-2527.
2、 Dolgachev VA, Yu B, Sun L,et al. Interleukin 10 overexpression alters survival in the setting of gram-negative pneumonia following lung contusion.[J].Shock,2014,41(4):301-310. Dolgachev VA, Yu B, Sun L,et al. Interleukin 10 overexpression alters survival in the setting of gram-negative pneumonia following lung contusion.[J].Shock,2014,41(4):301-310.
3、 Sugamata R, Dobashi H, Nagao T, et al. Contribution of neutrophil-derived myeloperoxidase in the early phase of fulminant acute respiratory distress syndrome induced by influenza virus infection [J].Microbiol Immunol,2012,56(3):171-182. Sugamata R, Dobashi H, Nagao T, et al. Contribution of neutrophil-derived myeloperoxidase in the early phase of fulminant acute respiratory distress syndrome induced by influenza virus infection [J].Microbiol Immunol,2012,56(3):171-182.
4、 Quinlan GJ,Evans TW,Gutteridge JM. Oxidative damage to plasma proteins in adult respiratory distress syndrome[J].Free Radical Res,1994,20(5):289-298. Quinlan GJ,Evans TW,Gutteridge JM. Oxidative damage to plasma proteins in adult respiratory distress syndrome[J].Free Radical Res,1994,20(5):289-298.
5、 Song J, Palmer K, Sun B. Effects of inhaled nitric oxide and surfactant with extracorporeal life support in recovery phase of septic acute lung injury in piglets[J].Pulm Pharmacol Ther,2010, 23(2): 78-87. Song J, Palmer K, Sun B. Effects of inhaled nitric oxide and surfactant with extracorporeal life support in recovery phase of septic acute lung injury in piglets[J].Pulm Pharmacol Ther,2010, 23(2): 78-87.
6、 Leone AM, Valgimigli M, Giannico MB, et al. From bone marrow to the arterial wall: the ongoing tale of endothelial progenitor cells [J]. Eur Heart,2009,30(8): 890-899. Leone AM, Valgimigli M, Giannico MB, et al. From bone marrow to the arterial wall: the ongoing tale of endothelial progenitor cells [J]. Eur Heart,2009,30(8): 890-899.
7、 Ito H,Matsushita S,Ishikawa S,et al. Significant correlation between endothelial nitric oxide synthase (eNOS) expression and alveolar repair in elastase-induced rat pulmonary emphysema [J]. Surg Today,2013,43(3): 293-299. Ito H,Matsushita S,Ishikawa S,et al. Significant correlation between endothelial nitric oxide synthase (eNOS) expression and alveolar repair in elastase-induced rat pulmonary emphysema [J]. Surg Today,2013,43(3): 293-299.
8、 Conese M1, Carbone A, Castellani S, et al. Paracrine effects and heterogeneity of marrow-derived stem/progenitor cells: relevance for the treatment of respiratory diseases.[J].Cells Tissues Organs,2013,197(6):445-73 Conese M1, Carbone A, Castellani S, et al. Paracrine effects and heterogeneity of marrow-derived stem/progenitor cells: relevance for the treatment of respiratory diseases.[J].Cells Tissues Organs,2013,197(6):445-73
9、 Mao M1, Xu X, Zhang Y, et al. Endothelial progenitor cells: the promise of cell-based therapies for acute lung injury [J]. Inflamm Res,2013,62(1):3-8. Mao M1, Xu X, Zhang Y, et al. Endothelial progenitor cells: the promise of cell-based therapies for acute lung injury [J]. Inflamm Res,2013,62(1):3-8.
10、 杨自力,王正国,朱佩芳.急性肺损伤早期NO的变化、意义及分子机制研究.第三军医大学学报[J].1995,17(6):517-518. 杨自力,王正国,朱佩芳.急性肺损伤早期NO的变化、意义及分子机制研究.第三军医大学学报[J].1995,17(6):517-518.
11、 Rubenfeld GD, Caldwell E, Peabody E,et al. Incidence and outcomes of acute lung injury [J].N Engl J Med,2005,353(16):1685-1693. Rubenfeld GD, Caldwell E, Peabody E,et al. Incidence and outcomes of acute lung injury [J].N Engl J Med,2005,353(16):1685-1693.
12、 Hasegawa M,Sakai S. Acute lung injury/acute respiratory distress syndrome: progress in diagnosis and treatment. Topics:II. Diagnosis and examination:1.Approach to diagnosis,2.Radiological findings of ALI/ARDS [J].Nihon Naika Gakkai Zasshi,2011,100(6):1545-1551. Hasegawa M,Sakai S. Acute lung injury/acute respiratory distress syndrome: progress in diagnosis and treatment. Topics:II. Diagnosis and examination:1.Approach to diagnosis,2.Radiological findings of ALI/ARDS [J].Nihon Naika Gakkai Zasshi,2011,100(6):1545-1551.
上一篇
下一篇
出版者信息








《广州医药》公众号
目录